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Molecular Diagnosis for Breast Malignancy.
Annual rept. 1 Jul 95-30 Jun 96,
GEORGETOWN UNIV WASHINGTON DC
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This grant investigates the role of EMMPRIN, seprase and DPPIV in breast cancer invasion and metastasis. The aims are to evaluate these conceivable invasion-related molecules as prognostic markers for node-negative breast cancer. We identified, cloned, and sequenced a full-length cDNA that encoded for a 58-kDa human tumor derived collagenase-stimulating factor, called EMMPRIN. We have expressed EMMPRIN in COS7 monkey kidney cells and human breast cancer cells in order to test the function of EMMPRIN as a collagenase-stimulating factor or a protease docking protein. To evaluate breast cancer markers, we have 1 established a new mAb screening assay of COS7 expression system for epitope mapping of mAbs, 2 improved the hybridoma protocol by including immunogens isolated directly from breast carcinoma tissues, 3 stained tumor sections of paraffin-embedded materials with nAb D8 and D28 against seprase, and 4 increased our efforts in developing serum tests for detecting breast cancer progression.
APPROVED FOR PUBLIC RELEASE