Developmental Toxicity Screen of Ammonium Dinitramide Using Hydra attenuata.
Final rept. Jun 95-Aug 96,
ARMSTRONG LAB BROOKS AFB TX OCCUPATIONAL AND ENVIRONMENTAL HEALTH DIRECTORATE
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The immediate application for the propellant ammonium dinitramide ADN is as a clean burning replacement for ammonium perchlorate in a formulation that also gains in performance. Currently, Air Force personnel are working with ADN during field testing trials. Reproductive toxicity evaluation of ADN through use of a modified Screening Information Data Set study using Sprague-Dawley rats produced adverse effects on litter parameters of treated animals. An in vitro developmental toxicity screen was performed to determine the developmental hazard index AD ratio for ADN using the hydra assay. Hydra attenuata have the capability for whole body regeneration. The assay employs exposing both adult hydra and artificial embryos composed of disassociated hydra cells to test compounds to investigate developmental toxicity. Artificial embryos are created by disassociating adult hydra into their component cells. Results from the hydra assay of ADN indicated the minimal effective concentrations required to elicit a toxic response in the adult hydra and in the regenerating hydra were 750 mg ADNL, respectively. The AD ratio of 2.14 determined for ADN demonstrates that ADN should not be considered a primary developmental toxic in the context of this assay.
- Environmental Health and Safety