Cutaneous Uptake of 14C-HD Vapor by the Hairless Guinea Pig.
Technical rept. Dec 92-Jun 93,
ARMY MEDICAL RESEARCH INST OF CHEMICAL DEFENSE ABERDEEN PROVING GROUND MD
Pagination or Media Count:
The hairless guinea pig HGP is used by our laboratory to model the human cutaneous response to sulfur mustard HD exposure. We have determined the HD content in the skin of HOP after 7-minute exposures to vapors saturated with a mixture of HD and 14C-HD. Concentrationtime C1 values in the range of 2 mgsq cmmin were determined by counting skin 14C disintegrations per minute dpm in animals euthanized immediately after exposure. These values are similar to human penetration rates obtained by other investigators. A direct relationship between C1 and relative humidity was demonstrated in 5 of 6 studies. A rate curve monitoring the reduction in skin 14C dpm was developed for animals euthanized between 0 and 24 hours post exposure. This curve showed the greatest change after 1 hour. Epidermal and dermal distribution of 14C at 24 hours was measured for two animals. Site preference for HD penetration, multiple use of a vapor cap containing HD, and 14C content of adhesive tape were also investigated with radiolabeled HD to evaluate other aspects of the experimental model. These results contribute to a better understanding of the cutaneous response to HD in the HGP model.
- Chemical, Biological and Radiological Warfare