A Novel Model System to Examine Agents Used in Breast Cancer Therapy.
Annual rept. 1 Jul 95-30 Jun 96,
MARYLAND UNIV BALTIMORE SCHOOL OF MEDICINE
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We have recently described a multiprotein complex for DNA replication DNA synthesome isolated from MDA MB 468 human breast cancer cells, human breast tumor tissue and human breast tumor cell xenografts In the presence of the viral large T-antigen and simian virus 40 SV40 origin sequences, the DNA synthesome executes all of the steps required for the in vitro replication of the SV40 genome. Furthermore, the DNA synthesome isolated from human breast cancer cells possesses a lower fidelity for DNA synthesis in vitro than the synthesome purified from a non-malignant breast cell line. Our studies indicate that the following DNA replication proteins constitute the DNA synthesome DNA polymerase alpha primase, DNA polymerase delta, DNA polymerase epsilon, proliferating cell nuclear antigen PCNA, RF-C, RP-A and DNA topoisomerases I, II.
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