Accession Number:

ADA318234

Title:

Structural and Functional Studies of Experimental HIV Synthetic Peptide Immunogens.

Descriptive Note:

Annual rept. 30 Sep 95-29 Sep 96,

Corporate Author:

DUKE UNIV MEDICAL CENTER DURHAM NC

Personal Author(s):

Report Date:

1996-10-01

Pagination or Media Count:

126.0

Abstract:

This grant addresses 2 major problems in HIV synthetic peptide vaccine development 1 the ability of synthetic peptides to mimic conformational determinants of HIV envelope proteins, and 2 the design of optimally immunogenic multivalent peptide immunogens capable of being recognized by MllC Class I and II types of outbred populations. In Technical Aim T.A. 1, a peptide mimitope of the 48d human mab has been found that binds to fusin T cells, and in binding to human cells, ceases exposure of the 48d binding site. Immunogenic gpi2O C4-V3 peptides have been mutated based on structural predictions that improve the immunogenicity of C4-V3 peptides. New potentially neutralizing determinant peptides from HIV gpi2O and gp4i have been made and are being tested in a novel water based adjuvant strategy utilizing intranasal immunization for optimal systemic antibody response generation. In T.A.2, the 4 C4-V3 peptides from HIV MN, RF, EV9i and CANOA as well as C4 mutant peptides have been studied using NMR, their solution conformers determined, and structure-function relationships made with studies in T.A.1. In T.A.3, an assay has been developed to predict HIV peptide binding to specific HLA molecules.

Subject Categories:

  • Biochemistry
  • Medicine and Medical Research
  • Pharmacology

Distribution Statement:

APPROVED FOR PUBLIC RELEASE