A Novel DNA-Based Vaccine Methodology for AIDS.
Annual rept. 30 Sep 95-29 Sep 96,
AGRACETUS INC MIDDLETON WI
Pagination or Media Count:
Gene gun-based DNA immunization studies in mice using an HIV-l gp120 expression vector demonstrated that modest effects on the strength and quality of gpl2O-specific immune responses could be elicited via the codelivery of vectors encoding murine IL-2, IL-7, or IL. 12. However, much more dramatic effects on gp 120-specific immune responses could be elicited by administering fewer immunizations over a longer time frame. In the nonhuman primate model, synergistic effects on the induction of HIV or SIV gpI2O-specific antibody titers were observed when gene gun immunizations were boosted with either recombinant subunit or recombinant vaccinia virus vaccines, In addition, a measurable vaccine effect was observed in rhesus macaques, in that lower virus loads and higher CD4 counts were observed in gene gun-vaccinated animals relative to naive controls following a heterologous challenge with SIVB67O. Protection was not correlated with the strength of gp120- specific antibody titers. In the swine model, very strong humoral responses were observed in a three dose regimen using as little as 0.5 ug of DNA per immunization. These responses were equivalent to those elicited following administration of a commercial adjuvanted recombinant subunit vaccine.
- Medicine and Medical Research