Regulation of Epidermal Growth Factor Receptor Expression by PML in Human Breast Cancer.
Annual rept. 1 Aug 95-31 Jul 96,
CANCER INST OF NEW JERSEY PISCATAWAY
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Expression of PML has been shown to be altered in breast cancer. In normal breast specimens, less than 3 of the epithelial cells exhibit PML staining, but increasing levels of PML was detected as the lesions progress from benign dysplasias to carcinomas. These studies suggest that PML may play a critical role in breast carcinogenesis. We have been studying the function of PML as a transcription regulator of the epidermal growth factor receptor EGFR gene. Recently, using a p21 promoter-reporter construct, we identified that the p21 gene is transcriptionally activated by PML, independent of p53 in a breast cancer cell line. These results suggest that PML may be one of the transcription regulators that mediates p21 gene expression. Current work in the lab focuses on characterization of PML expression by immunohistochemistry in paraffin embedded sections derived from patients breast cancer samples.
- Genetic Engineering and Molecular Biology
- Anatomy and Physiology
- Medicine and Medical Research