Role of a Novel Antioncogene that Prevents Metastatic Spread of Disease.
Annual rept. 1 Jul 95-30 Jun 96,
BOWMAN GRAY SCHOOL OF MEDICINE WINSTON-SALEM NC
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The purpose of this grant is to determine a mechanism of action for a novel antioncogene i.e. nm23 which prevents the metastatic spread of disease. Our data would indicate that there is no relationship of nm23 expression with proteolytic factors such as cathepsin D, urokinase plasminogen activator, its receptor and its inhibitor. High levels of these factors have been shown to predict metastatic disease in breast cancer patients. Both western blot and ELISA analyses and also by immunohistochemistry were used to prove this lack of correlation. We did show that nm23 expression was related to metastatic potential using cell lines and xenographs. We also performed a clinical trial to determine if nm23 could predict prognosis in node negative breast cancer patients. Our results indicate that nm23 is not a independent prognostic indicator. Our results do indicate that when MDA-MB-23 1 cells are transfected with nm23 gene, they have a lower motility and become less metastatic in the nude mouse model system.
- Medicine and Medical Research