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Retrieval of Water Channels by Endocytosis in Renal Epithelia.

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Annual rept. 5 Jun 95-4 Jun 96,

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Combat crew effectiveness is crucial during sustained and demanding military operations. In particular, how soldiers cope with conditions of excess heat and fluid deprivation will ultimately impact on their ability to carry out their stated objectives. If the kidney should become damaged or obstructed, the kidney will fail to respond to the hormone vasopressin, which is responsible for regulating water balance. Understanding the specific mechanisms involved in the regulation of water balance by the kidney will provide information needed to design preventive measures for dealing with potential adverse conditions that may result in water deprivation and decreased renal responsiveness to vasopressin. The present study is designed to determine the cellular mechanisms involved in reduced fluid reabsorption and membrane reorganization following the removal of the actions of ADH. It is proposed that following removal of hormone, proteinaceous water channels, located in the apical membrane, are internalized through a process of endocytosis, delivered to the eytosol as a consequence of changes in intracellular calcium concentrations, PKC enzyme activation and reorganization of the microfilamentmicrotubule system. These studies may lead to new drug or non-drug treatment regimes that could be used to enhance the kidneys responsiveness to vasopressin or to maintain the level of water reabsorptive capacity of soldiers facing harsh environmental conditions of prolonged exposure to dehydration and water deprivation or impaired renal function from injury. Such preventive measures will be essential for maintaining the performance and combat effectiveness of our military forces.

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  • Medicine and Medical Research

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