Effect of Hormone Replacement Therapies and Dietary Phytoestrogens on the Mammary Gland of Macaques.
Annual rept. 1 Jul 95-30 Jun 96,
BOWMAN GRAY SCHOOL OF MEDICINE WINSTON-SALEM NC
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The purpose of this study was to use histomorphometric and immunohistochemical techniques to study the incidence and characteristics of mammary gland hyperplasia, dysplasia and other possible indicators of breast cancer risk, in cynomolgus macaques given long-term hormonal treatments. Treatments evaluated to date include conjugated estrogens CEE, medroxyprogesterone acetate MPA, the combination of CEE and MPA, tamoxifen, estradiol E2, 17 alpha-dihydroequilenin DHEN, soybean phytoestrogens SBE, and SBE E2. Pathologic evaluation, histomorphometric evaluations, and staining for estrogen receptor, progesterone receptor, and the proliferation marker Ki-67 MIB were done. Results are as follows The addition of MPA to CEE therapy increases, rather than decreases, mammary gland proliferation. Tamoxifen treatment does not induce mammary gland proliferation beyond that seen in controls this is in contrast to a marked uterotrophic effect. DHEN does not induce mammary gland or endometrial proliferation, relative to controls and in contrast to E2. Soybean estrogens do not induce mammary or endometrial proliferation when given alone, and exert an antagonistic effect on E2-induced proliferation in both sites. New work includes dietary modulation of-hormonal effects on mammary gland, identification of p53 expression in CEE-treated macaque mammary gland, and development of whole-mount techniques.
- Medicine and Medical Research