Mapping of a Breast Carcinoma Tumor Suppressor Gene to Chromosome 11p15.5.
Annual rept. 1 Jul 95-30 Jun 96,
NORTH CAROLINA UNIV AT CHAPEL HILL
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Loss of heterozygosity LOH on the short arm of chromosome 11 has been demonstrated in many cancers, suggesting the presence of a tumor suppressor gene. A number of studies have narrowed the region showing LOH in breast tumors to 11p15.5. The technical objectives of this study are to screen breast tumor samples for LOH in the 11p15.5 region using markers in the region exhibiting the greatest LOH to further resolve the location of the putative tumor suppressor gene and to screen candidate genes for abnormal expression in breast tumor samples. In the three months since reactivation of the fellowship, the collection of breast tumor DNA samples has been expanded. We have used these samples to begin our candidate gene analysis. Currently, we are working with p57KIP2, NAP2, and TAPA1. Following our candidate gene analysis, we plan to study the region of interest using polymorphic markers to look for LOH in the breast tumor DNA. We have selected marker which fall near the contig map that has been constructed in our laboratory. We will start with existing markers to define the area exhibiting LOH and then further delineate the region with new markers.
- Medicine and Medical Research