Mapping Mammary Carcinoma Suppressor Genes in the Laboratory Rat.
Annual rept. 1 Jul 95-30 Jun 96,
Pagination or Media Count:
Inbred strains of rats differ in their susceptibility to both spontaneous and chemically-induced mammary carcinoma formation. We are currently using molecular biology techniques combined with classical genetic breeding studies to try to identify the mammary carcinoma suppressor MCS genes responsible for the mammary carcinoma resistance trait in inbred Copenhagen Cop and Wistar Kyoto WKy rats. Simple sequence repeat SSR markers from the literature as well as from our chromosome specific libraries were used to map the rat genome and fine-map the regions that involved in the resistance phenotype. Previous data identified a quantitative trait locus QTL on rat chromosome 2 which is linked to the resistance phenotype the gene is termed Mcs-1, and a marker on rat chromosome 7 which also appeared linked to the resistance phenotype the gene is termed Mcs-2. Since the last progress report, 27 additional markers have been placed on the chromosome 2 map. One marker, CA2.F1, increased the LOD score to 4.154, further confirming the QTL at Mcs-1. Thirteen additional markers have been placed on the chromosome 7 map, and Mcs-2 is localized near a region on chromosome 7q13. Moreover, we found two other regions which show suggestive linkage to the resistance phenotype, one each on chromosomes 1 and 7.
- Medicine and Medical Research