Physiology, Biochemistry, and Genetics of a Pure Culture of an Obligatory Anaerobic Bacterium That Utilizes 2,4,-6-Trinitrotoluene (TNT) and Biodegradation of RDX by Pure Cultures of Obligatory Anaerobic Bacteria of the Genus Clostridium.
Final rept. 1 Sep 93-31 Aug 96,
IDAHO UNIV MOSCOW CENTER FOR HAZARDOUS WASTE REMEDIATION RESEARCH
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In work supported by the US AFOSR grant F49620-94-1-0306 we are conducting detailed biochemical and genetic studies of three strains of Clostridium bifernientans, obligatory anaerobic bacteria that appear to completely degrade a variety of nitroaromatic compounds, including 2,4,6-trinitrotoluene TNT. We are determining the optimal physiological conditions for the degradative activities of C. bifermentans strains and identifying and characterizing enzymes and genes involved in the biotransformation of nitroaromatic compounds by C. bifermentans. In our AASERT supplemental grantAFOSR-93-1-O464 we expanded these goals to the explosive RDX 1,3,5-triaza-1,3,5-trinitrocyclohexane. The AASERT grant funded two graduate students, who characterized the ability of C. bifermentans to degrade RDX Regan, K. N., and R.L. Crawford, 1994. Biotechnol. Kett. 16 1081- 1086, and prepared both genomic and plasmid DNA libraries from C. bifermentans. This genetic work will accelerate our progress toward our goal of characterizing the genetics of TNTRDx degradation by our clostridia K. Diedrich, M.S. thesis, University of Idaho in preparation.
- *NITROGEN COMPOUNDS
- DEOXYRIBONUCLEIC ACIDS
- PHYSIOLOGICAL EFFECTS
- AROMATIC COMPOUNDS
- ANAEROBIC BACTERIA
- ANAEROBIC PROCESSES
- Ammunition and Explosives