Circadian Rhythms in Zebrafish.
Final rept. 1 Aug 94-31 Jul 96,
KANSAS UNIV MEDICAL CENTER KANSAS CITY
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The goal of this project was to determine whether the zebrafish would be a useful system for genetic analysis of the cellular and molecular mechanisms that comprise vertebrate circadian clocks. An understanding of biological clock mechanisms will aid in the treatment of the performance deficits, sleep disorders and other problems associated with jet lag, shift work and organic clock deficits in humans. Experiments were performed to characterize behavioral, physiological and molecular circadian rhythms that can be used to screen for genetic clock mutations and to investigate the roles of mutant genes. Robust circadian rhythms of behavior in both larval and adult zebrafish were found and characterized. In addition, the exceptionally strong circadian rhythms of melatonin release by cultured zebrafish pineal glands were characterized. The results indicate that genetic analysis of vertebrate clock mechanisms will be feasible using this model system.
- Anatomy and Physiology