Chronic Toxicity Studies on 1,3,5-Trinitrobenzene in Fischer 344 Rats.
Final rept. 1 May 93-30 Apr 95,
ENVIRONMENTAL PROTECTION AGENCY CINCINNATI OH RISK REDUCTION ENGINEERING LAB
Pagination or Media Count:
Chronic toxic effects of I ,3,5-trinitrobenzene TNB in male and female Fischer rats were evaluated by feeding certified powdered laboratory chow diet supplemented with varied concentrations of TNB 0, 5, 60 and 300 mgkg diet. The study was designed to accommodate three interim sacrifices 10 ratsgroupsex at 90, 180 and 365 days. The final sacrifice was performed after two years. All data related to these interim sacrifices are presented independently in appendices J to L. The calculated average TNB consumption for females was 0.23, 2.68 and 13.31 mgkgday and was 0.22, 2.64 and 13.44 mgkgday for males. Terminal body weights were significantly decreased in both sexes in the 300 mgkg group. Relative spleen weights were decreased in both sexes in the 300 mgkg group while brain weights were increased in females in this same group. Methemoglobin was increased in both sexes in the 300 mgkg group while other hematological effects noted at the interim sacrifice times were not evident at two years. Histopathological examinations suggested treatment related changes in both sexes involving the kidneys cytoplasmichyaline droplets in the 60 and 300 mgkg groups and the spleen erythroid cell hyperplasia and pigment deposition in the 300 mgkg group. The cytoplasmichyaline droplets were characterized by immunohistochemistry as alpha-2u-globulin. These renal droplets were also noted at the interim sacrifice times. A no observed adverse effect level NOAEL was established in this study at 2.68 mgkg b.w.day for F-344 rats administered TNB for two years.
- Inorganic Chemistry