Studies on Human Breast Cancer and Transforming Growth Factor-beta Application for a Career Development Award.
Annual rept. 1 Oct 94-30 Sep 95,
DARTMOUTH COLL HANOVER NH
Pagination or Media Count:
This is a career development award. The primary focus of the research is the expression and function of TGF-Beta by breast cancer cells. For established tumors, overexpression of TGF-B may result in increased in vivo tumor growth and metastatic spread. The first objective involves the analysis of resected breast cancer specimens, with the goal of confirming our prior work correlating clinical outcome with TGF-B expression levels in tumors. There are as yet no definitive data or conclusions from this aspect of the work. The second objective of the grant was to evaluate the impact of TGF-Beta on the tumorigenic and metastatic potential of human breast cancer cell lines in nude mice. We have made progress on generating the transfected cell clones, but do not yet have data regarding animal studies. The third research objective is the analysis of the regulation of TGF-Beta expression in breast cancer cells. We have made some progress in designing required promoter constructs for this work, but do not have data as yet.
- Genetic Engineering and Molecular Biology
- Medicine and Medical Research