Wnt-5a and Wnt-4 Regulates Cell Growth in C57MG Mammary Epithelial Cells.
Annual rept. 1 Jul 95-30 Jun 96,
PENNSYLVANIA STATE UNIV UNIVERSITY PARK
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The implications for determining whether wnt-5a and wnt-4 act as mediators of normal cell growth are relevant to the detection, diagnosis, and the treatment of breast cancer. That is, it is important ultimately to understand whether the inappropriate downregulation of certain wnt-genes that are spatially-temporally expressed in developing mammary glands, such as wnt-5a and wnt-4, leads to loss of growth control. C57MG mammary epithelial cells have recently been found to endogenously express wnt-5a and wnt-4 whose RNA levels are reduced in the presence wnt-gene family members which transform this cell line. The intent of this proposal has been to develop new cell lines by transfecting C57MG cells with DNA wnt-5a andor wnt-4 constructs which express antisense or sense RNA to determinethe effect on cell transformation will occur. Results found in experiments outlined in this proposal are consistent with the importance for normal wnt-5a andor wnt-4 RNA transcripts levels in directing C57MG mammary epithelial cell phenotype, including cell proliferation and transformation. This has important ramifications for breast cancer since it is known that wnt-5a and wnt-4 genes are downregulated in human breast cancer tissue and in human breast malignant cell lines.
- Medicine and Medical Research