Accession Number:

ADA310710

Title:

Cholinesterase Structure: Identification of Residues and Domains Affecting Organophosphate Inhibition and Catalysis.

Descriptive Note:

Annual rept. 6 Mar 95-5 Mar 96,

Corporate Author:

CALIFORNIA UNIV SAN DIEGO LA JOLLA

Personal Author(s):

Report Date:

1996-04-01

Pagination or Media Count:

124.0

Abstract:

In the initial year of the grant, we have made excellent progress in several arenas 1 A crystal structure of a mouse acetylcholinesterase-fasciculin 2 complex has been solved. 2 Studies with enantiomeric organophosphates have yielded vital information on their binding orientation in the ground and transition states. 3 Studies in oxime reactivation of inhibited cholinesterase have uncovered the basis for enhanced reactivity of HI-6 compared to 2-PAM. 4 The interactions of fasciculin 2 with acetylcholinesterase have been studied by kinetic and site-specific mutagenesis methods.

Subject Categories:

  • Biochemistry
  • Pharmacology

Distribution Statement:

APPROVED FOR PUBLIC RELEASE