Cholinesterase Structure: Identification of Residues and Domains Affecting Organophosphate Inhibition and Catalysis.
Annual rept. 6 Mar 95-5 Mar 96,
CALIFORNIA UNIV SAN DIEGO LA JOLLA
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In the initial year of the grant, we have made excellent progress in several arenas 1 A crystal structure of a mouse acetylcholinesterase-fasciculin 2 complex has been solved. 2 Studies with enantiomeric organophosphates have yielded vital information on their binding orientation in the ground and transition states. 3 Studies in oxime reactivation of inhibited cholinesterase have uncovered the basis for enhanced reactivity of HI-6 compared to 2-PAM. 4 The interactions of fasciculin 2 with acetylcholinesterase have been studied by kinetic and site-specific mutagenesis methods.