Accession Number:

ADA305656

Title:

Crystallization and Structure Determination of the Human Estrogen Receptor by X-ray Diffraction.

Descriptive Note:

Annual rept. 15 Sep 94-14 Sep 95,

Corporate Author:

CHILDREN'S HOSPITAL MEDICAL CENTER BOSTON MA

Personal Author(s):

Report Date:

1995-10-01

Pagination or Media Count:

18.0

Abstract:

The project is a postdoctoral training fellowship for Dr. Robert Nolte, to support X-ray crystallographic studies of cancer-related macromolecular complexes. 1 We have expressed various constructs of the human estrogen receptor DNA-binding domain, but failed to obtain crystallizable protein. Success of other groups in this and related efforts have led us to focus on different goals. 2 We have prepared crystals of a complex between a six Zn-finger segment of the transcription factor TFIIIA and a 31 bp DNA site. This structure will reveal significant aspects of DNA recognition by Zn-finger proteins, which regulate many loci important for cell growth. 3 During the coming project year, Dr. Nolte will embark on structural studies of Src-related tyrosine kinase activity, as described in the body of the progress report

Subject Categories:

  • Biochemistry
  • Medicine and Medical Research
  • Crystallography

Distribution Statement:

APPROVED FOR PUBLIC RELEASE