Crystallization and Structure Determination of the Human Estrogen Receptor by X-ray Diffraction.
Annual rept. 15 Sep 94-14 Sep 95,
CHILDREN'S HOSPITAL MEDICAL CENTER BOSTON MA
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The project is a postdoctoral training fellowship for Dr. Robert Nolte, to support X-ray crystallographic studies of cancer-related macromolecular complexes. 1 We have expressed various constructs of the human estrogen receptor DNA-binding domain, but failed to obtain crystallizable protein. Success of other groups in this and related efforts have led us to focus on different goals. 2 We have prepared crystals of a complex between a six Zn-finger segment of the transcription factor TFIIIA and a 31 bp DNA site. This structure will reveal significant aspects of DNA recognition by Zn-finger proteins, which regulate many loci important for cell growth. 3 During the coming project year, Dr. Nolte will embark on structural studies of Src-related tyrosine kinase activity, as described in the body of the progress report
- Medicine and Medical Research