Protein Neo-Antigens in Breast Cancer by Combinatorial Phage Technology.
Annual rept. 23 Sep 94-22 Sep 95,
NEW ENGLAND DEACONESS HOSPITAL BOSTON MA
Pagination or Media Count:
IT IS Intended to identify and characterize newly expressed proteins neo-antigens which elicit plasma cell reactions in medullary and in non-medullary ductal carcinomas with circumscription and plasma cell infiltration. To accomplish this purpose, we exploit recently developed molecular procedures in a new application to derive antibodies from the tumor-infiltrating plasma cells, and then use these antibodies to retrieve the proteins whose new expression in the tumors Induced the response. Ig sequencing of two patient MC samples revealed reiteration, supporting the presence of a focussed, specific immune response against an antigen by reactive plasma cells In the MC tumor. Additionally, phage Fab clones from these combinatorial phage libraries were shown to bind to HTB24 cells, the only available MC cell line, confirming cell surface expression of the putative neo-antigens on these cells. The neo-antigens will be identified by immunoprecipitation with reactive antibodIes. Subsequently these proteins will be assessed for possible roles in the malignant proliferation and as subjects for anti-breast cancer interventions.
- *MAMMARY GLANDS
- *ADRENAL MEDULLA HORMONES
- *BREAST CANCER
- SURFACE PROPERTIES
- COMBINATORIAL ANALYSIS
- Anatomy and Physiology
- Genetic Engineering and Molecular Biology
- Medicine and Medical Research