Genetic Toxicity Evaluation of 1,3,3-Trinitroazetidine. Volume 4. Summary report on the Genotoxicity of TNAZ.
Final rept. Jul 92-Dec 93,
TOXIKON CORP WOBURN MA
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In this study, 1,3,3-trinitroazetidine TNAZ, a potential component of Air Force explosive formulations, was examined for mutagenic and clastogenic activity. The mutagenic activity of TNAZ was evaluated by both the Salmonella Microsome Reverse Mutation Assay and the Chinese Hamster Ovary CellHypoxanthine-Guanine Phosphoribosyl Transferase CHOHGPRT Forward Mutation Assay. TNAZ, in the presence and absence of 5-9 microsomal enzymes, failed to induce histidine reversion his- to his by base changes or frameshift mutation in the genome of five strains of Salmonella typhimurium bacterial. Furthermore, TNAZ, with and without 5-9 metabolic activation, failed to induce a significant increase in mutant colonies at the HGPRT locus in cultured CR0 cells. Additional independent confirmatory Ames and CHOHGPRT assays indicated TNAZ was non-mutagenic. TNAZ was also examined for its effect on the chromosomes and mitotic apparatus of albino Swiss mice bone marrow cells by measuring micronuclei formation in maturing erythrocytes. The mouse bone marrow micronucleus test measures the clastogenic potential of chemicals in vivo. The number of micronucleated cells in the maturing erythrocytes in the bone marrow cells of mice was not increased in the presence of TNAZ but was increased greater than 30 fold by mitomycin C. In conclusion, under the test systems and conditions employed in this study, TNAZ is considered non-mutagenic and non-clastogenic.
- Genetic Engineering and Molecular Biology