Genetic Toxicity Evaluation of 1,3,3-Trinitroazetidine. Volume 1. Results of Salmonella Typhimurium Reverse Mutation Assay.
Final rept. Jul-Dec 92,
TOXIKON CORP WOBURN MA
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The Salmonella typhimurium reverse mutation assay Ames assay evaluated the potential of 1 3,3-trinitroazetidine TNAZ to induce histidine his reversion his to his, caused by base changes or frameshift mutations in the genome of this organism. This direct plate incorporation assay was conducted with five strains of Salmonella typhimurium, in the presence and absence of an exogenous mammalian activation system. The preincubation technique was used to enhance the sensitivity 0 the plate incorporation assay. The range finding study was conducted with and without metabolic activation to determine levels at which TNAZ exhibited toxicity. The reverse mutation assay determined the test substance to be non-mutagenic. The results of the assay were confirmed through an independent confirmatory assay.
- Genetic Engineering and Molecular Biology