Genetic Toxicity Evaluation of 1,3,3-Trinitroazetidine. Volume 3. Results of Gene Mutation at the HGPRT Locus in Cultured Chinese Hamster Ovary Cells.
Final rept. Jul-9 Dec 92,
TOXIKON CORP WOBURN MA
Pagination or Media Count:
The test substance, 1, 3,3-Trinitroazetidine TNAZboth in the presence and absence of microsomal S-9 liver enzyme, failed to induce a significant increase in mutant colonies at the HGPRT locus in cultured Chinese hamster ovary cells. The effects of TNAZ at the dose levels selected based on the initial range finding assay were similar to those of the negative control incomplete Hams F 12 medium and solvent control dimethylsulfoxide substances. Both positive control substances 4-nitroquinoline- I-oxide and dimethylnitrosamine in the presence and absence of an activation system, exhibited a significantly increased induction of mutant colonies. Confirmatory assays were performed utilizing the same test system conditions as the initial assays. The results were similar and consistent with the initial mutagenicity assay. The data indicates that TNAZ did not induce an increased number of mutants compared to the negative and solvent control substance values. Therefore, TNAZ is considered non-mutagenic under the conditions utilized in this study.
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