Advanced Research Training in Marine Molecular Biology and Biotechnology.
Final rept. 1 May 92-30 Apr 94,
DUKE UNIV BEAUFORT NC MARINE LAB
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The ONR sponsored grant entitled Advanced Research Training in Marine Molecular Biology and Biotechnology founded a training course to teach students and established scientists the basic principles of modern recombinant DNA technology. The emphasis was on the development of marine models and problems in environmental and ocean sciences. Students were trained in the isolation and characterization of DNA and RNA which included Southern and Northern blotting, DNA sequencing and cDNA libraries. Other techniques taught were the use of the polymerase chain reaction PCR, in situ hybridization, gene transfer, mtDNA analysis and immunoblotting. These techniques were applied to problems in environmental toxicology, marine microbiology, the development of transgenic organisms, evolution and population studies, developmental biology and gene expression analysis.
- *DEOXYRIBONUCLEIC ACIDS
- *MARINE BIOLOGY
- *MOLECULAR BIOLOGY
- *RIBONUCLEIC ACIDS
- *MARINE CORPS TRAINING
- GENETIC ENGINEERING
- CHAIN REACTIONS
- Genetic Engineering and Molecular Biology
- Biological Oceanography