The Role of Prostaglandins in the Growth of Breast Epithelial Cells.
Annual rept. 15 Aug 94-14 Aug 95,
UTAH UNIV SALT LAKE CITY
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The growth of mammary epithelial cells in response to growth factors is augmented by linoleic or arachidonic acid. This may explain the association between dietary fatty acid intake and increased risk of breast cancer. We are exploring the mechanism by which these fatty acids exert this growth-promoting effect with the central hypothesis that oxidative metabolism is a crucial step. Moreover, we are testing the hypothesis that induction of prostaglandin H synthase is required. In the first year we have shown that this enzyme is induced in breast epithelial cells in response to growth factors, and have found that the growth-promoting effect of fatty acids largely, but not completely, requires conversion to prostaglandins. The additional effect appears to be mediated via activation of specific isotypes of protein kinase C. We are in the process of additional testing of the role of key metabolic enzymes with antisense knockout of individual candidates.
- Medicine and Medical Research
- Anatomy and Physiology