Tissue Banks and Cell Lines Derived from Patients with a High Risk of Breast Cancer and In Situ Disease.
Annual rept. 1 Nov 94-31 Oct 95,
ROYAL CANCER HOSPITAL LONDON (UNITED KINGDOM) INST OF CANCER RESEARCH
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This project is aimed at establishing bulk cultures of individual epithelial and stromal cells from mastectomy specimens of patients who are carrying genes that predispose them to breast cancer. In addition epithelial and stromal cells are being conditionally immortalized using a novel amphotropic retroviral vector transducing the tsA58-U1 9 SV4O-derived construct. These cells are from patients with a range of predisposing phenotypes which include BROAl, BROA2, Li- Fraumeni and ataxia telangiectasia. In parallel a detailed data base is being established of Th situ disease from patients that have been sent to the Royal Marsden Hospital Breast Unit since 1970. To date samples have been received from 21 women with a strong family history, six of which have a Li-Fraumeni phenotype and the rest are from families that are presumptive carriers of BROAl or BROA2 mutations. In the majority of cases primary cultures have been established and cells banked for future immortalization. Over 5,000 slides have been reviewed of in situ breast cancer from over 600 cases. Material from 468 patients has been selected for further study. Sections are being cut for collaborative studies and paraffin blocks have been identified that are suitable for molecular analyses.
- Medicine and Medical Research
- Anatomy and Physiology