Accession Number:

ADA303514

Title:

X-Ray Crystallography of Botulinum Neurotoxins.

Descriptive Note:

Annual rept. 1 May 93-30 Sep 95,

Corporate Author:

VETERANS ADMINISTRATION MEDICAL CENTER PITTSBURGH PA

Personal Author(s):

Report Date:

1995-10-01

Pagination or Media Count:

7.0

Abstract:

Botulinum neurotoxin, type E Bot E was purified to evaluate the affects of purity on crystallization. It was found that purification of the product from PHLSCAMR Proton, Salisbury, VK did not improve crystal quality significantly. Accordingly, the product was used as supplied in further crystallization experiments. Diffraction quality crystals were grown by VAMC personnel at Fort Detrick, but the crystal which were transported in sealed capillary tubes deteriorated in transportation. To overcome this problem, a safe and secure laboratory was set up in the Pittsburgh VAMC UD in compliance with Federal regulations to handle Bot E neurotoxin. Crystals were grown which diffracted to 3.A, and a set of intensity data was collected from it. Work is progressing with the aim of getting more mature and derivative data.

Subject Categories:

  • Biochemistry
  • Radiobiology
  • Crystallography

Distribution Statement:

APPROVED FOR PUBLIC RELEASE