The Role of G2/M Checkpoint Controls in Cytotoxic Treatment of Breast Cancer.
Annual rept. 15 Aug 94-14 Aug 95,
SCRIPPS RESEARCH INST LA JOLLA CA
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The experiments described are based on the hypothesis that the development and progression of breast cancer is associated with alterations in the operation of cell-cycle checkpoints that normally maintain the fidelity and coordination of cell division and that these alterations determine the susceptibility of cells to cytotoxic therapy This research is intended to provide a detailed understanding of the action of cytotoxic anti-cancer agents and therefore addresses the issue of how treatment of breast cancer can be improved. Using the techniques of biochemistry and molecular biology we have examined the pattern of expression and activity of mitotic checkpoint control proteins CDC2, Cyclin B, Cyclin A in transformed and non-transformed breast cells. These results suggested that would be necessary to directly control the level of expression of CDC2,Cyclin B,WEEl and CDC25 by transfection in order to determine the importance of checkpoint control proteins in cytotoxic sensitivity. These experiments, which are expected to provide evidence of a causal relationship between changes in cell cycle control proteins and the efficacy of cytotoxic therapy, are currently in progress when - these experiments are completed we will examine the response of transformed and non-transformed breast cell lines to cytotoxic treatments.
- Medicine and Medical Research
- Anatomy and Physiology