Characterization of Breast Cancer Progression by Analysis of Genetic Markers.
Annual rept. 30 Sep 94-29 Sep 95,
AMERICAN REGISTRY OF PATHOLOGY WASHINGTON DC
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Genetic changes implicated in the etiology of breast cancer have been identified by the detection of loss of heterozygosity at specific loci. Our study utilizes a series of genetic polymorphisms detectable by the polymerase chain reaction PCR to look for changing patterns of LOH as breast cancer progresses from intraductal to metastatic disease. The initial phases of this work involved the identification of cases from the AFIP archives, microdissection of individual tumor components, and PCR amplification with multiple markers. To date, 248 cases have been identified for the study. Lysates have been prepared from 93 cases. Oligonucleotides were synthesized to detect the nine polymorphisms targeted for the initial screen in the grant proposal. Additional markers were identified and tested to determine ones that would be useful for the study. The 93 cases for which lysates have thus far been prepared have been analyzed for LoH at two loci on chromosome lip15. The results demonstrate our ability to evaluate individual tumor components for LOH and validate the general approach, which will be used extensively in the next phase of the project.
- Medicine and Medical Research
- Genetic Engineering and Molecular Biology