Mechanisms of Integrin-Mediated Growth Control in Normal, Transformed, and Neoplastic Breast Cells.
Annual rept. 30 Sep 94-29 Sep 95,
SEATTLE BIOMEDICAL RESEARCH INST WA
Pagination or Media Count:
The interaction between a normal cell and its substratum, a phenomenon known as anchorage dependence, is an important determinant of the G IS transition. The cell surface receptors that mediate cell-substrate adhesion are known as integrins. It is possible that extracellular matrix proteins regulate normal cell growth by transmitting signals to the interior via specific integrin receptors. in transformed cells such signaling pathways might be absent or dysfunctional thereby conferring unresponsiveness to normal growth constraints. The present studies are focused to understand how integrin receptors, in particular a5B1, participate in the regulation of cell division in normal breast cells and to determine how breast cancer cells escape these regulatory pathways. Our preliminary findings demonstrate that ligation of the a5B1 integrin receptor in normal breast cells by URUDS peptides results in the rapid activation of cdc2 a cyclin dependent kinase active at the Oils transition. These findings provide the first clear evidence in support of a direct role for integrin receptors in the control of normal cell division. The effects of a5B1 mediated signaling in transformed BC will be investigated.
- Anatomy and Physiology
- Medicine and Medical Research