Identifying and Isolating Breast Cancer Associated Genes on Chromosome 11.
Annual rept. 1 Oct 94-30 Sep 95,
HEALTH RESEARCH INC BUFFALO NY
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A complete YAC yeast artificial chromosome contig is being constructed across a defined region in lip15.5 associated with breast cancer using our chromosome ii YAC library. Candidate genes will be identified by exon amplification and cDNA enrichment. Chromosome rearrangements in this region will be utilized for mapping and isolation of tumor suppressor genes. Breast cancer tissue will be examined by Southern blot and ribonuclease protection analysis to identify possible tumor suppressor genes. Candidate genes will be analyzed using single strand conformational analysis SSCA and chemical mismatch cleavage CMC to identify mutations. These studies should provide insights into the genetic mechanisms involved in breast cancer development and diagnostic tools for subclassifying breast tumors with alterations at lip15 and, therefore, be of prognostic value.
- Anatomy and Physiology
- Genetic Engineering and Molecular Biology
- Medicine and Medical Research