Role of Changes in the Expression of Cyclins and Retinoblastoma Protein in the Development of Breast Cancer.
Annual rept. 23 Sep 94-22 Sep 95,
COLORADO UNIV AT DENVER
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In the initial year of the grant, we have carried out the bulk of the studies to characterize breast cancer cell lines with respect to overexpression of cyclin Dl protein and the presence of Rb retinoblastoma protein Specific Aim No. 1. In addition, we have begun examination of surgically obtained breast cancer tissue samples for cyclin Dl protein overexpression and Rb protein content Specific Aim No. 6. Since the original grant proposal was submitted, advances in our understanding of cell cycle regulation, and in particular advances in our understanding of the importance of defects in cell cycle regulation to the development of cancer, have proceeded at a rapid pace . In order to incorporate these advances into our investigation of cell cycle regulatory defects in breast cancer, we have expanded and reoriented some aspects of our approach. This has included a detailed assessment of the levels of cyclin dependent kinase cdk 4 and cdk 6, and of the cdk4cdk6-specific cdk inhibitor protein pl6 see Results below. This reorientation of the investigation in the light of ongoing research advances can be accommodated within the framework of the originally requested funds and resources.
- Medicine and Medical Research
- Genetic Engineering and Molecular Biology