Breast Cancer in Ataxia Telangiectasia Carriers.
Annual rept. 15 Aug 94-14 Aug 95,
M D ANDERSON CANCER CENTER HOUSTON TX
Pagination or Media Count:
This proposal seeks to determine if the ataxia-telangiectasia AT gene acts as a tumor suppressor gene in a subset of breast cancers by looking for tumor loss of constitutional heterozygosity at chromosomal region 11q22-q23, the map position of the AT genes. The technical aims are to analyze 300 matched pairs of tumor and normal tissue from breast cancer patients for tumor loss of constitutional heterozygosity using a panel of amplification polymorphisms mapping to 11q22-q23 and, when loss of heterozygosity is detected, to map the extent of that loss. Over the past year, 37 sets of tumor and normal tissue in pathology storage as paraffin embedded samples were screened for 14 polymorphic loci based on simple sequence repeats SSRs using the polymerase chain reaction PCR. Seventeen of these tumors were found to have lost heterozygosity either in the region of the AT gene or an adjacent region that might overlap. All but one of these deletions appear to be interstitial.
- Anatomy and Physiology
- Medicine and Medical Research