Establishment of a Repository for Cell Cultures and Genomic DNA from Breast Cancer Patients.
Annual rept. 1 Sep 94-31 Aug 95,
BIOLOGICAL RESEARCH FACULTY AND FACILITY INC IJAMSVILLE MD
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New breast cancer cell strains and cell lines were developed from many breast cancer tissues and from uninvolved normal tissues. These cell strainslines were added to all-new cell repository for breast cancer cells and genomic DNA. Primary cultures were initiated using cell suspensions isolated from the tissues and using cellular outgrowth from explanted tissues. Various cell isolation methods and a variety of culture media and hormone combinations were tested to avoid senescence in vitro. Thirty cultures passed through the second subculture and ten of these could be expanded to a sufficient number for cryopreservation. Of these ten, six may be classified as cell strains and the other four as cell lines since they were continuously cultured for over ten subcultures. The cell lines have been partially characterized and all possess H-ras and p53 genes. Additional cells will be developed and characterized during the next grant period.
- Anatomy and Physiology
- Medicine and Medical Research