Tumor Suppressor Genes in Early Breast Cancer and Its Progression.
Annual rept. 1 Sep 94-31 Aug 95,
WASHINGTON UNIV ST LOUIS MO SCHOOL OF MEDICINE
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Ductal carcinoma in situ DCI S of the breast is a pre-invasive form of breast cancer, which in some cases is a precursor to invasive ductal cancer. Many tumor suppressor loci are inactivated in invasive breast cancer, however little is known concerning the genetic events preceding invasion. Using polymerase chain reaction of microsatellite markers we have allelotyped 61 tumornormal pairs from patients with DCIS. Tumor DNA template was obtained from microdissected archival samples. Significant loss of heterozygosity LOll was found for chromosomal loci on 8p 18.7, 13q 18, 16q 28.6, 17p 37.5 and 17q 15.9. The region of allelic loss on 8p has been examined with an expanded panel of markers on 64 tumors, and the smallest region of deletion narrowed down to 1.3 cM. A fine structure map of 8p is being created using genotypic data from CEPH families. Assay of twelve sets of synchronous in situ and invasive breast cancer revealed that clonal expansion takes place in the progression of DCIS and that loci on lip may be involved on the invasive phenotype.
- Anatomy and Physiology
- Genetic Engineering and Molecular Biology
- Medicine and Medical Research