Developing a System for Directed Gene Introduction into Mammary Gland Via Targeted Infection of Retrovirus Receptor Transgenics.
PENNSYLVANIA UNIV PHILADELPHIA
Pagination or Media Count:
This work describes the development of a novel system for efficient and rapid introduction of genes into specific tissues or cells in mice i.e. in vivo targeted gene expression. Detailed analysis of gene function often relies upon introduction of genes into an animal model system. We are developing a retroviral-based system in which controlled expression of the cellular receptor for the virus in a defined pattern determines the sites of infection. Using this retroviral gene delivery system, a gene can be introduced not only in a spatial-specific pattern but also in a temporally- controlled manner. For these studies we will express the virus receptor in mammary cells in transgenic mice using a MMTV LTR. The ability to selectively target retroviral infection has numerous applications in areas as diverse as developmental biology, gene therapy and oncogenesis. Here we will attempt to utilize this system for targeted expression of genes in mammary cells in an animal model.
- Anatomy and Physiology
- Medicine and Medical Research