Effect of HSP27 on Human Breast Tumor Cell Growth and Motility.
Annual rept. 1 Sep 94-31 Aug 95,
NEVADA UNIV RENO
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This award is a Predoctoral Fellowship to support the doctoral training of Donna Egender. The goal of this research is to investigate the effects of the small stress protein, hsp27, on growth and motility characteristics of normal and tumor derived human mammary cell lines. Our study is based on the hypothesis that hsp27 is component of a signal transduction pathway that regulates actin microfilament dynamics, and may affect cell migration and the metastatic potential of tumors. We hypothesize that cells overexpessing hsp27 will show increased motility and altered chemotactic properties, in addition to increased resistance to heat killing and certain drugs. Academically, Donna has finished her formal course requirements, and successfully completed the written and oral Doctoral Preliminary Exam. Research completed this year includes preparation of constitutive and inducible expression vectors for overexpressing human hsp27 and mouse hsp25 in transfected human mammary cells. An antisense construction for downregulation of human hsp27 expression has also been repared. Preliminary experiments to optimize transfection of human MCF7 and MDA23 1 cells have been performed, as well as experiments to gain facility in determining clonal survival of stress.
- Anatomy and Physiology