Accession Number:

ADA300584

Title:

Effect of Transforming Growth Factor Beta (TGF Beta) and Vitamin D3 Metabolites on Protein Kinase C Mediated Signal Transduction in Rat Costochondral Chondrocyte Cultures.

Descriptive Note:

Master's thesis,

Corporate Author:

TEXAS UNIV AT SAN ANTONIO GRADUATE SCHOOL OF BIOMEDICAL SCIENCES

Personal Author(s):

Report Date:

1995-05-01

Pagination or Media Count:

103.0

Abstract:

It is Imown that vitamin D3 metabolites and transforming growth factor TOF-J3 regulate proliferation and differentiation of chondrocytes isolated from the growth zone or resting zone of rat costochondral cartilage. It was hypothesized that both TGF- and the vitamin D metabolites play a role in signal transduction in the rat costochondral chondrocyte model and that the regulation of protein kinase C by these metabolites would be cell maturation-dependent. Confluent, fourth passage cultures of growth zone and resting zone chondrocytes were treated with vitamin D3 metabolites for up to 24 hours, lysed, and the cell extracts assayed for protein kinase C PKC specific activity using a specific peptide substrate. The addition of i,25-OH2D3 to growth zone chondrocyte cultures resulted in a rapid, dose dependent stimulation of PKC specific activity.

Subject Categories:

  • Biochemistry
  • Medicine and Medical Research

Distribution Statement:

APPROVED FOR PUBLIC RELEASE