Accession Number:

ADA300581

Title:

Programmed Cell Death in Breast Cancer.

Descriptive Note:

Annual progress rept. 1 Oct 94-30 Sep 95,

Corporate Author:

CASE WESTERN RESERVE UNIV CLEVELAND OH

Personal Author(s):

Report Date:

1995-08-28

Pagination or Media Count:

16.0

Abstract:

E HAVE INITIATED STUDIES OF PROGRAMMED CELL DEATH, OR POPTOSIS, IN HUMAN BREAST CANCER CELLS. Using the MCF-V reast cancer cell line, we have found that cell growth is stimulated by supplementing the culture medium with l-estradio1, and inhibited by charcoal stripping serum o remove steroid hormones. We have found that MCF- cells are resistant to induction of apoptosis by the calcium pump inhibitorr thapsigargin, ostensibly due to expression of Bcl-2. We have found that estrogen ithdrawal decreases the level of Bcl-2 expression, but does not increase the susceptibility of cells to apoptosis due to constitutive Bcl-2 expression.

Subject Categories:

  • Anatomy and Physiology
  • Medicine and Medical Research

Distribution Statement:

APPROVED FOR PUBLIC RELEASE