Accession Number:
ADA300572
Title:
Novel Approaches to the Characterization of Specific Protein-Protein Interactions Important in Gene Expression.
Descriptive Note:
Final technical rept.,
Corporate Author:
PURDUE RESEARCH FOUNDATION LAFAYETTE IN DIV OF SPONSORED PROGRAMS
Personal Author(s):
Report Date:
1995-10-14
Pagination or Media Count:
7.0
Abstract:
Through the application of a range of biochemical, immunochemical and genetic techniques, we have accomplished the following goals First, we characterized a previously undescribed protein, WrbA and showed that it functions as an accessory protein in the binding of TrpR to its operator target second, we demonstrated that WrbA is a stationary-phase protein, and that the regulation of promoters controlled by TrpR becomes more stringent in stationary phase cells third, we developed a method for the purification of the TyrR protein, a dual-specificity transcription factor that can both activate and inhibit the action of RNA polymerase. The domain structure of TyrR was established by chemical procedures.
Descriptors:
Subject Categories:
- Genetic Engineering and Molecular Biology
- Medicine and Medical Research