Accession Number:

ADA300424

Title:

Evaluation of the Metabolic Fate of Munitions Material (TNT & RDX) in Plant Systems and Initial Assessment of Material Interaction with Plant Genetic Material.

Descriptive Note:

Final rept. 8 Feb 93-31 May 95,

Corporate Author:

DEPARTMENT OF ENERGY RICHLAND WA RICHLAND OPERATIONS OFFICE

Report Date:

1995-06-01

Pagination or Media Count:

48.0

Abstract:

The goals of this effort were to confirm and expand data related to the behavior and impacts of munitions residues upon human food chain components. Plant species employed included corn Zea mays, alfalfa Medicago sativa, spinach Spinacea oeraceae, and carrot Daucus carota. Plants were grown from seed to maturity 70 to 120 days in a low-fertility soil Burbank amended with either 14C-TNT or 14 C-RDX at which time they were harvested and analyzed for munitions uptake, partitioning, and chemical form of the munition or munition-metabolite. All four of the plant species used in this study accumulated the 14C-TNT- and RDX-derived label. The carrot, alfalfa, and corn demonstrated a higher percentage of label retained in the roots 62, 73, and 83 respectively. The spinach contained less activity in its root 36 but also contained the highest TNT specific activity observed 4600 ug TNT- equivalentsg dry wt. respectively. The specific uptake values of RDX for the spinach and alfalfa were comparable to those previously reported for wheat and bean 314 to 5g0 ug RDX-equivalentsg dry wt. respectively. An exception to this may be the carrot where the specific activity was found to exceed 4200 ug RDX-equivalentslg dry wt. in the shoot. The total accumulation of TNT by the plants ranged from 1.24 for the spinach to 2.34 for the carrot. The RDX plants ranging from 15 for the spinach to 37 for the carrot. There was no identifiable TNT or aminodinitrotoluene ADNT isomers present in th plants however, the parent RDX compound was found at significant levels in the shoot of alfalfa 180 ugg and corn 18 ugg.

Subject Categories:

  • Biochemistry
  • Medicine and Medical Research
  • Ammunition and Explosives

Distribution Statement:

APPROVED FOR PUBLIC RELEASE