Accession Number:

ADA300118

Title:

New Therapeutic Approaches and Prognostic Assays for Breast Cancer: Radiolabeled Ligans and Antibodies and Quantitative PCR.

Descriptive Note:

Annual rept. 30 Sep 94-29 Sep 95,

Corporate Author:

HOWARD UNIV WASHINGTON DC COLL OF MEDICINE

Personal Author(s):

Report Date:

1995-08-28

Pagination or Media Count:

14.0

Abstract:

Our research proposal is on developing highly sensitive, rapid, cost-effective molecular assays for quantitating the transcripts of prognostic factors in breast cancer. In the first granting period, our goals were to establish the conditions for quantitating the transcripts of wild type-, and variant types of estrogen receptor ER using breast cancer cell lines in culture. We have designed PCR primers which can amplify various portions of ER mRNA. We tested the primers first on a plasmid cDNA plC-ER-F which contains full length coding sequences of ER to establish the optimum PCR conditions. We have then used them to amplify various exons of ER cDNA prepared by reverse transcribing the RNA from MCF-7 cells. We are currently in the process of quantitaing the ER transcripts in MCF-7 cells by competition assay.

Subject Categories:

  • Medicine and Medical Research
  • Anatomy and Physiology
  • Inorganic Chemistry

Distribution Statement:

APPROVED FOR PUBLIC RELEASE