Accession Number:

ADA300054

Title:

Genomic Instability at Premalignant and Early Stages of Breast Cancer Development

Descriptive Note:

Annual rept. 1 Aug 1994-31 Jul 1995

Corporate Author:

TEXAS UNIV AT HOUSTON

Personal Author(s):

Report Date:

1995-08-31

Pagination or Media Count:

35.0

Abstract:

We have developed techniques that allow the analysis of multiple chromosomal loci from single paraffin sections from breast cancer lesions. This approach is being used to allelotype small preinvasive breast cancer lesions, correlating with other parameters such as specific gene mutations and expression and with interphase cytogenetics on the same lesions. We identified the chromosome arms most frequently affected by allelic losses and imbalances at preinvasive stages of breast carcinogenesis and those allelic losses involved in more advanced stages of progression. We are continuing with these studies and further refining the mapping of specific subchromosome areas involved in early breast tumorigenesis. In the course of these studies we observed that microsatellite instability replication error phenotype, RER is characteristic of a subset of breast tumors invasive lobular breast carcinomas, indicating the possible involvement of defective DNA mismatch repair genes in these tumors. Studies are ongoing to address this point and to evaluate the use of RER phenotype determination as a prognostic-diagnostic tool.

Subject Categories:

  • Medicine and Medical Research

Distribution Statement:

APPROVED FOR PUBLIC RELEASE