Molecular Determinants of Antiestrogen and Drug Sensitivity in Breast Carcinoma Cells.
Annual rept. 15 Jul 94-14 Jul 95,
ILLINOIS UNIV AT CHICAGO CIRCLE
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NCF7 breast carcinoma cells were rendered susceptible to infection with recombinant ecotropic retroviruses, through transfection with the gene for murine ecotropic receptor and by testing individual transfectants for infectability. A genetic suppressor element GSE, derived from the BCL2 gene which inhibits active cell death apoptosis, was introduced into NCF7 cells. The BCL2-derived GSE sensitized MCF7 cells to different chemotherapeutic drugs, indicating that BCLt2 is an important determinant of chemoresistance of these cells. A retroviral library of normalized cDNA fragments, derived from Heta cell line, was introduced into NCF7 cells, and selection of GSEs that render MCF7 cells resistant to doxorubicin was initiated. A normalized cDNA library is also being prepared from NCF7 cells, for use as a source of GSEs conferring resistance to tamoxifen.
- Medicine and Medical Research
- Anatomy and Physiology