Accession Number:

ADA300046

Title:

Molecular Determinants of Antiestrogen and Drug Sensitivity in Breast Carcinoma Cells.

Descriptive Note:

Annual rept. 15 Jul 94-14 Jul 95,

Corporate Author:

ILLINOIS UNIV AT CHICAGO CIRCLE

Personal Author(s):

Report Date:

1995-08-14

Pagination or Media Count:

20.0

Abstract:

NCF7 breast carcinoma cells were rendered susceptible to infection with recombinant ecotropic retroviruses, through transfection with the gene for murine ecotropic receptor and by testing individual transfectants for infectability. A genetic suppressor element GSE, derived from the BCL2 gene which inhibits active cell death apoptosis, was introduced into NCF7 cells. The BCL2-derived GSE sensitized MCF7 cells to different chemotherapeutic drugs, indicating that BCLt2 is an important determinant of chemoresistance of these cells. A retroviral library of normalized cDNA fragments, derived from Heta cell line, was introduced into NCF7 cells, and selection of GSEs that render MCF7 cells resistant to doxorubicin was initiated. A normalized cDNA library is also being prepared from NCF7 cells, for use as a source of GSEs conferring resistance to tamoxifen.

Subject Categories:

  • Medicine and Medical Research
  • Anatomy and Physiology
  • Pharmacology

Distribution Statement:

APPROVED FOR PUBLIC RELEASE