Genetic Analysis of Human Breast Cancer.
Annual rept. 15 Jul 94-14 Jul 95,
COLD SPRING HARBOR LAB OF QUANTITATIVE BIOLOGY NY
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Cancer is a disorder brought upon by the accumulation of specific mutations in the cancerous cells. Our understanding of the disease, and potentially its diagnosis and therapeutic treatment, is enhanced by defining the genetic lesions that cause it. Representational difference analysis, or RDA, was developed to do just this. RDA is a DNA subtraction methodology that finds sequences present in one DNA population, the tester, that is absent or reduced in a second, the driver 1, 2. RDA has been used to discover sequences lost or amplified in the genomic DNA of the cancerous cells 3. Genetic loss and gene amplification are hallmarks of tumor suppressor genes and oncogenes, respectively. We have been applying RDA to the discovery of sequences lost in breast cancer. At least three loci undergoing loss in breast cancer have been identified. The transcriptional potential of these loci is being explored. Characterization of many other RDA probes is in progress. We expect that the continued execution of our stated plan will accomplish our stated goal, the identification of tumor suppressor genes that are commonly involved in breast cancer.
- Medicine and Medical Research
- Genetic Engineering and Molecular Biology