Role of High-Affinity Oncostatin M Receptor in Prevention of Breast Cancer Cell Growth.
Annual rept. 9 May 94-31 Mar 95,
VETERANS ADMINISTRATION MEDICAL CENTER BOISE ID
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To investigate the anti-tumor activity of Oncostatin M against breast cancer cells, we examined the effect of OM on the growth of several human breast cancer cell lines that were established from solid tumors or malignant effusions removed from patients. Cellular proliferation assays indicated that the growth of 7 out of 9 cell lines was inhibited by 50-90 compared with untreated cells by picomolar concentrations of OM. In comparison with OM, IL-6 and TGF-Beta only slightly inhibited the proliferation of these cells. The effects of OM on anchorage-independent cell growth were also tested. Both the number and the size of the colonies formed by ZR-75-1 cells, one of the breast cancer cell lines, were reduced by OM in a soft agar assay. The facts that OM did not totally inhibit DNA synthesis, and that following removal of OM cells continued to grow at a reduced rate, suggest that OM may induce a differentiation process in these malignant cells. The radioligand receptor binding indicated that all 7 breast cancer cell lines responding to OM possess high-affinity OM receptors. The OM non-responsive cell lines lack specific binding sites for OM.
- Medicine and Medical Research