Role of Proteoglycans in Cellular Signaling.
Final rept. 1 Oct 90-31 Sep 93,
MEHARRY MEDICAL COLL NASHVILLE TN
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Proteoglycans have the ability to regulate the activity of several growth factors important to wound healing, including basic fibroblast growth factor and transforming growth factor-beta. In this project, we surveyed proteoglycan synthesis in a number of strains of normal human skin fibroblasts and in fibroblast strains derived from benign dermal tumors keloids which show a prolonged wound healing response and abnormal responses to hydrocortisone in tissue culture. Anion exchange chromatography was used to separate radiolabeled proteoglycans into heparan sulfate and dermatan sulfate charge classes. The most consistent and dramatic trend observed for all strains was the apparent hydrocortisone-induced decrease of dermatan sulfate proteoglycan accumulation in the cell-associated fraction from normal strains but not from keloid strains. Experiments are in progress to measure file messenger RNA for several dermatan sulfate proteoglycan core proteins known to be synthesized by human skin fibroblasts. Since the data collected so far are still incomplete, we intend to finish this project by measuring message levels for these core proteins mentioned above. Once these experiments are completed, we plan to publish these data and conclude this project.
- Anatomy and Physiology
- Medicine and Medical Research