Comparison of Commercial Colorimetric and Enzyme Immunoassay Field Screening Methods for TNT in Soil. Installation Restoration Research Program.
ARMY ENGINEER WATERWAYS EXPERIMENT STATION VICKSBURG MS
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A study comparing two commercially available methods of field screening for TNT in soil, utilized 99 soil samples from the Naval Surface Warfare Center, Crane, Indiana. All soil samples were analyzed using a commercial colorimetric method EnSys and a commercial enzyme immunoassay method D ThCH, and the results were compared with those from the standard reversed-phase high performance liquid chromatography RP-HPLC laboratory method SW846 Method 8330. Comparisons were made relative to numerical agreement of screening results with laboratory analysis by RP-HPLC and usage in two distinct scenarios. Of the 99 soil samples analyzed by the laboratory method, 25 had TNT 2,4,6-trinitrotoluene concentrations greater than the RP-HPLC detection limit of 0.3 ugg. Of these 25, 11 had concentrations in the range 0.3- 1.0 ugg and 14 had concentrations greater than 1.0 ugg the operating field screening detection limit for this study. Results were positive from both field screening methods for all 14 soils with TNT concentrations greater than 1.0 ugg by RP-HPLC. Thus, no false negatives were observed by either method for samples above the field screening detection limit. Of the 11 samples with TNT concentrations in the range 0.3 - 1.0 ugg, D ThCH failed to detect 3 samples and EnSys failed to detect 2 samples.
- Geology, Geochemistry and Mineralogy
- Ammunition and Explosives