Development of an Aquatic Bioassay using the Medaka (Oryzias latipes) to Assess Human Health Risk: Tumor Immunodiagnosis.
Final rept. 28 Sep 90-27 Feb 94,
JOHNS HOPKINS UNIV BALTIMORE MD SCHOOL OF MEDICINE
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In a continuation project for the development of aquatic bioassays as alternatives for carcinogenicity and toxicity testing to assess human health risk, immunohistochemical techniques IHC for the diagnosis of carcinogen induced neoplasms and other proliferative lesions in fish will be developed. Groups of medaka Oryzias latipes have been exposed to either N-methyl-N-nitro-nitrosoguanidine MNNG or methylazoxymethanol acetate MAN-Ac to induce variable neoplasms and proliferative lesions to which IHC can be applied to identify differentiation antigens. Both peroxidase-antiperoxidase and avidin biotin complex techniques are used to identify intermediate filament proteins keratin, desmin, vimentin, glial fibrillary acidic protein, and neurofilament protein. Other selected antigens may also be tested for based on histology. These findings will be correlated with histology, and ultrastructure. The results of this proposal will identify association between cell types in neoplasms and in proliferative lesions of uncertain origin which cannot yet be types by morphology alone in the fish, and thereby identify the possible progression of these lesions to neoplasms. These data are essential to the development of aquatic bioassays to evaluate human health risk and thus this research will contribute significantly to the development of the model.
- Medicine and Medical Research