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Vasopressin Receptor Signaling and Cycling of Water Channels in Renal Epithelia.
Final rept. 1 Aug 91-31 Jul 94,
JARVIS CHRISTIAN COLL HAWKINS TX
Pagination or Media Count:
Water reabsorption and urinary volume regulation are important processes in maintaining normal physiological function. Vasopressin ADH is the hormone that is responsible for regulating water homeostasis and enhancing water reabsorption during dehydration. This process is particularly important to soldiers who are subject to excess heat and fluid deprivation in arid environments. We have been examining the mechanisms whereby ADH enhances water permeability in renal epithelia so we may find measures to enhance the kidneys responsiveness to ADH. Such regimens would maintain the water reabsorptive capacity of soldiers facing harsh conditions. Our observations suggest that an integral component of the water reabsorptive process by ADH is the enzyme, protein kinase C PKO and calcium mobilization in addition to ADHs well established actions on cyclic AMP. Using immunocytochemical and fluorescent techniques we have identified PKC isozymes that translocate from the cytosol to the membrane during activation by ADH or PKC activators. We also have developed in vitro cultured cell model that responds to vasopressin that could be used to study transepithelial fluid and electrolyte transport and have evaluated various cultured cell filter supports for their transport characteristic limitations.
APPROVED FOR PUBLIC RELEASE